elisa standard curve fit

trevor g · External Since: Jan 30, 2007 Posts: 1

I am a biochemist and I am wanting to be able to plot immunoassay (ELISA)
data. In particular I want to be able to produce standard curves using a
4-parameter logistic curve fit and read the values of 'unknown' samples from
the curve.

Bernard Liengme · External Since: Jan 27, 2004 Posts: 2613

I can show you how to fit a logistics curve using Solver - Write to my
private email - I
Also try Google with <ELISA curve> and with <ELISA logistic Excel>
best wishes
--
Bernard V Liengme
www.stfx.ca/people/bliengme
remove caps from email

"trevor g" wrote in message

>I am a biochemist and I am wanting to be able to plot immunoassay (ELISA)
> data. In particular I want to be able to produce standard curves using a
> 4-parameter logistic curve fit and read the values of 'unknown' samples
> from
> the curve.

assay wiz · External Since: Feb 02, 2012 Posts: 1

Try http://www.myassays.com/four-parameter-fit.assay

This has everything you need to do a 4PL with standards and unknowns and it's free. Also, there is an Add-in for Excel available at http://www.myassays.com/introducing-excel-add-in.html

> On Tuesday, January 30, 2007 5:55 AM trevor wrote:

> I am a biochemist and I am wanting to be able to plot immunoassay (ELISA)
> data. In particular I want to be able to produce standard curves using a
> 4-parameter logistic curve fit and read the values of 'unknown' samples from
> the curve.

>> On Tuesday, January 30, 2007 12:43 PM Bernard Liengme wrote:

>> I can show you how to fit a logistics curve using Solver - Write to my
>> private email - I
>> Also try Google with <ELISA curve> and with <ELISA logistic Excel>
>> best wishes
>> --
>> Bernard V Liengme
>> www.stfx.ca/people/bliengme
>> remove caps from email
>>
>> "trevor g" wrote in message
>> news:43CB2C23-F29F-4BF9-B36B-74C1BBCEED12@microsoft.com...

Charles Ma · External Since: Feb 03, 2012 Posts: 1

Hi Trevor,

I know this a "bit" late as a reply. But I hope this can help you and others who landed here. We have a FREE, easy to use online tool for ELISA analysis at www.readerfit.com. It is able to fit your standard curve with 4-parameter logistic and 5-parameter logistic as well. If you come to our site, we have great email/live support where we can walk you through a typical analysis.

Charles Ma
Technical Support
Hitachi Solutions America, Ltd.
MiraiBio Group
Website: http://www.MiraiBio.com

===
Check out our new online free ELISA analysis tool at http://www.ReaderFit.com
===

ericvandamme18 · External Since: Apr 22, 2012 Posts: 1

Hi Trevor,

I think IVDfit may be something for you. IVDfit is an Excel add-in. It can do 4PL and 5PL and it is specially made for ELISA data. You can also calculate your unknowns with it. It can be downloaded at www.ivdtools.com